Hebin Liu

Qualifications

Ph.D.   Umeå University,  Umeå, Sweden,  2005

M.Sc.   Zhejiang University,  Hangzhou, China,  Immune Biochemistry,  1996

B.Sc.   Zhejiang University,  Hangzhou, China,  Veterinary Medicine,  1993


Experience

Faculty member, Department of Biological Science, XJTLU (September, 2012 - Present), Suzhou, China-PRC.

Senior Investigator, Department of Pathology, University of Cambridge (October, 2009 - September, 2012), Cambridge, United Kingdom.

Research associate, Department of Pathology, University of Cambridge (February, 2006 - November, 2009), Cambridge, United Kingdom.

Postdoctoral Research associate, Department of Molecular Immunology, Imperial College London (March, 2005 - February, 2006), London, United Kingdom.

Lecturer, Centre for Disease Control and Preventions of Zhejiang Province (1996 - 2000), Unknown, China-PRC


Professional Service Activities 

Reviewer - Grant Proposal Related to Expertise

2014:  UK funding body BBSRC (International).


Invited Lecture

2013:  3rd avian pathogens and immunology conference organised by Chinese Agricultural Academy of Sciences, Shanghai (International).   keynote talk: Immune adaptors and T cell signalling. 

2012:  EUROPEAN CONGRESS OF IMMUNOLOGY, Glasgow, Scotland (International).  Immune adaptor SLP-76 regulation of GAP activity of RanGap1 at nuclear pore complex (NPC) for T cell activation.

2008:  Keystone Symposium on Lymphocyte Activation and Signaling, Snowbird, Utah, United States of America (International).  SUMO modification regulates TCR signalling.

2007:  Department of Pathology Retreat, King's college, University of Cambridge, Cambridge, United Kingdom (International).  SLP-76 sumoylation plays a central role in micro-cluster formation and signalling in T-cells. 

2007:  13th CLSS-UK (Chinese Life Science Symposium-UK) meeting, Cambridge, United Kingdom (International).  SUMO modification regulates TCR signalling.


Professional Memberships 

British Society for Immunology (BSI), 2014.

Research Interests 

Research Interests

Our laboratory investigates the molecular basis and regulation of T cell activation, focusing on the functional roles of immune adapter proteins e.g. SLP-76 in TCR signalling.

Topics of on-going projects in the lab and collaborations include:

1. Crosstalk between the post-transcriptional modification.SUMO pathway and immune adaptors in the T cell signalling. While ubiquitination down-regulates signaling thresholds in T-cells and autoimmunity, a role for SUMOylation in T-cell activation has been missing. The goal of this study is to identify T cell specific substrates of SUMO pathway in T cell activation and investigate the role of SUMOylation in immune adaptor-mediated TCR signaling and T cell activation. This study will provide a direct insights into the functional relations between the post-transcriptional SUMO modification and T cell activation. Ultimately, it is our hope that these studies may lead to the development of pharmacological interventions and clinical strategies for treatment of immune-related diseases by control of T-cell functions through manipulation of the level of SUMO modified form of a T specific molecule such as immune adapter proteins.

 2. Host T cell immunity-pathogen interaction: Despite major advances in the understanding of virus–host cellular interactions, the mechanisms of viral escape from T cell immunity and the underlying pathogenic mechanisms remain elusive.  Our goals are to gain a better understanding of how a persistent viral infection is established and to devise novel tools to promote T cell immunity in immunocompromised individuals. We are interested in fundamental aspects of how host immune adapter-mediated T cell signalling and immune responses are regulated and affected  in response to some animal virus (e.g., PCV2 infection) , including microcluster formation of immune adapters, T cell-APC recognition, proximal and distal events of TCR signalling.


Publications 

Liu, H., Schneider, H., Recino, A., Richardson, C., Goldberg, M., CE Rudd (2015).  The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells.   Molecular Cell, 59 (5), 840-9 (Cover story).


Smith, H. X., Schneider, K., Kohler, H., Liu, H., Lu, Y., C.E. Rudd. (2013).  The Chemokine CXCL12 Generates Costimulatory Signals in T Cells to Enhance Phosphorylation and Clustering of the Adaptor Protein SLP-76.   Science (Signal), 6 (286), ra65.


Liu, H., Raj, T., Loren, S., Helga, S., John, E., Ladbury and Christopher E. Rudd (2013).  SLP-76 sterile alpha motif (SAM) and individual H5 alpha helix mediates oligomer formation for microclusters and T-cell activation.   J Biol Chem, 288 (41), 29539-49.


Purbhoo, M.A, Liu, H., Oddos, S., Owen, D. M., Neil, M. A., S.V. Pageon, P.M. French, C.E. Rudd, and D.M. Davis. (2010).  Dynamics of subsynaptic vesicles and surface microclusters at the immunological synapse.   Science (Signal), 3 (121), ra36.


Liu, H., Purbhoo, M. A., Davis, D. M., & Rudd, C. E. (2010).  SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering.   Proc Natl Acad Sci USA (107), 10166-1017.


Schneider, H., Smith, X., Liu, H., Bismuth, G., & Rudd, C. E. (2008).  CTLA-4 disrupts ZAP-70 microcluster formation with reduced T-cell/APC dwell times and calcium mobilization.   Eur J Immunol, 38 (1), 40-7.


Wang, H., Liu, H., Lu, Y., Lovatt, M., Wei, B., Rudd, C. E (2007).  Functional defects of SKAP-55 deficient T-cells identify a regulatory role for the adaptor in LFA-1 adhesion.   Mol Cell Biol, 27 (9), 6863-75.


Liu, H., Carlsson, L., & Grundstrom, T. (2006).  Identification of an N-terminal transactivation domain of Runx1 that separates molecular function from global differentiation function.   J Biol Chem (281), 25659-69.


Duke-Cohan, S. J., Kang,, H., Liu, H., & Rudd, E. C. (2006).  Regulation and function of SKAP-55 non-canonical motif binding to the SH3c domain of adhesion and degranulation-promoting adaptor protein.   J Biol Chem (281), 13743-50.


Liu, H., Holm, M., Xie, X., Wolf-Watz, M., & Grundstrom, T. (2004).  AML1/Runx1 recruits calcineurin to regulate granulocyte macrophage colony-stimulating factor by Ets1 activation.   J Biol Chem (279), 29398-408.


Liu, H. & Grundstrom, T. (2002).  Calcium regulation of GM-CSF by calmodulin-dependent kinase II phosphorylation of Ets1.   Mol Biol Cell (13), 4497-507.


Research Grants 

Research

2015 [Year 1 of 3]: Liu, H. Regulation of T cell receptor (TCR) signaling by SUMOylation of immune adaptor SLP-76 in T cells-NSFC general program, Principal Investigator, GOV-National Science Foundation (NSF).


2013 [Year 2 of 3]: Liu, H. An investigation on the regulation of T cell activation by SUMOylation of immune adaptor SLP-76, Principal Investigator, Jiangsu Science and Technology Programme.


2013 [Year 2 of 3]: Liu, H. An investigation into the role of SUMOylation pathway in T-cell signaling, Principal Investigator, XJTLU Research Development Fund.

1997: Liu, H. Studies on Expression of Nucleocapsid Protein (NP) of Hantavirus Vaccine Strain Z10 in E.coli and Applications of the Recombinant NP in Diagnosis, Principal Investigator, Zhejiang Science and Technology Programme.